Reagents and plate setting

 

 

Slot :

 

 

Phasis :

ORIGIN ELU D1 E D2 SP W1 W2 W3 µMB DeepWell including samples on Thermoshake  

 

 

Empty 96-wells Deepwell

(And binding columns plate depending on installed technology)

Mix Tubes on Inheco with tubes adapter

 

qPCR plate on Inheco with pcr adapter

 

Extraction

ADNucleis_EXTRACT.gem or ADNucleis_EXTRACT_DIL*

Clean Waters

 

90 mL* 30 mL

 

15 mL  

X

Extraction Purification

ADNucleis_EXTRACT_PURIF.gem

All other matrices 15 mL 30 mL 15 mL Cf ADN_SOFT Cf ADN_SOFT Cf ADN_SOFT Cf ADN_SOFT Cf ADN_SOFT  

X

(The plate contains samples inside 100µL F and 100µL PVG buffers)

X
Extraction Purification COLUMNS

ADNucleis_COL_EXTRACT_PURIF.gem

All other matrices 15 mL 30 mL Cf ADN_SOFT Cf ADN_SOFT Cf ADN_SOFT Cf ADN_SOFT  

X

(The plate contains samples inside 100µL F and 100µL PVG buffers)

Seal partially the silica columns plate where unused wells :

 

 

 

X

PCR plate preparation

ADNucleis_qPCR_plate_prep.gem

Extract w/wo Purif 15 mL 30 mL  

X

(The plate contains eluted DNA Deepwell)

 

X

(cf program for ref and positions)

 

X

Posted in Technical notice.