Real Time PCR as an alternative to cultural methods

Real-time PCR is known to allow the isolation, identification and quantification of the DNA of bacteria, viruses, parasites, allergens and GMOs in a very short time (1 to 3 hours) in all areas: food safety, animal and / or human health.

For a long time, this technic was of limited use due to the cost of the detection of some food hygiene related pathogens, the short shelf life of some products, the goal of Professor Michel Franck, CEO ADNucleis, is to expand the application of this PCR technic to the identification and quantification of traditional hygiene indicators (TVC, E.coli, coliforms, Pseudomonas spp, Listeria spp), and / or pathogens (Salmonella, Listeria monocytogenes, Cronobacter sakazakii, E. coli STEC O157H7, viruses , parasites …). This goal has now been achieved, thanks to a new innovative method, at equivalent prices to the prices of traditional cultural methods.

This method, especially when checking for routine contaminants on a production line, can be done with or without a specialized robotic platform, also developed by ADNucleis.

Thanks to its industrial partners, ADNucleis continues its goal to make real-time PCR an industry standard though the simplification and automation of the entire analytical process.

PCR Kit Cronobacter spp (Enterobacter sakazakii)

An AFNOR certified Cronobacter spp (Enterobacter sakazakii) Real Time PCR kit is available at ADNucleis.

ADNucleis has positioned itself in Food quality and hygiene market for the fast testing of milk powder and infant formula with its Cronobacter spp (Enterobacter sakazakii) PCR kit.

The harmful effect of this bacteria on at risk individuals is well known. In France, the numerous cases of food poisoning, in the 90s early 2000s, with an infant mortality rate, among those affected, of about 40 to 80% are proof of these harmful effects.

ADNucleis is AFNOR certified for its diagnostic product, Cronobacter spp (Enterobacter sakazakii) NF VALIDATION 16140.

Like all ADNucleis PCR kits, the analysis is fast (less than 24 hours, including pre-enrichment) and the cost is particularly competitive since ADNucleis has reduced its price to that of culture method namely 2€ per amplification kit (all real-time PCR platforms are compatible with this analysis)

Operator time is very short and can be fully automated, which gives PCR an undeniable competitive advantage; it is estimated at less than 2 minutes per sample; the rest of the time is instrument time (55mn).

Contact communication :

Virginie Morel

R&D communication

v.morel@adnucleis.com

Automatisation

ADNucleis provides automation, for PCR sample prep, with features including the following:

  • DNA and RNA extraction and purification with silica microbeads or columns,
  • Plate preparation, for an input of 1 to 3 sample plates and an output of up to 3 PCR plates,
  • Shaking, cooling and heating steps.

Thanks to its innovative algorithms and washing processes, ADNucleis platforms perform protocols with a decontamination step in order to avoid the change of tips. The process launches automatically when needed, the user-friendly software allows the operator to selects the specific(s) target(s) for which his sample is being prepared, and the robot does the rest.

ADNucleis’ extraction and purification protocols are functional with all robotic pipetting platforms with all the benefits of robotics: repeatability of operations, quality and traceability.

We provide the choice of 2 methods: the faster method with disposable tips (1 minute 30 per sample) or more economically advantageous, without disposable tips with our needle decontamination protocol (3 minutes per sample)

This protocol is compatible with large throughput (up to 100 000 extractions per year per unit).

ADNucleis provides the protocols for extraction and purification by silicon beads or columns, and for the preparation of PCR plates.

Our easy to use, innovative algorithms, allows the operator to easily prepare up to 3 PCR plate from plates of extracted DNA or RNA, with a needle decontamination step with each column change.