Troubleshooting – Robot

In case of error, initialization error, please check that there was no collision done by a bad positioning of tube, rack, plates… As the robot initialize its position on each start, it is possible to clear problem by switching off the robot (wait 3 sec) and re switching on. Restart Gemini and initialization.


« Device is not implemented », happening during initialization

Cause :

Can be caused by a wrong initial position which causes an mechanical problem.

 

Corrective Task :

Turn off Pipetting Robot, move the Robotic Arm on any another position of X, Y and rotation, then re launch Pipetting Robot and Gemini software.


Diluter error “tip is broken”

Cause : This problem can occur when a wrong dispense operation has been done. The robot or the operator “Desactivated “ the tip.

Corrective Task : 

To repair this, Setup -> Configuration -> LiHa -> correct the check box where the tip is marked as broken.


Diluter error “Plunger overload”

The robot stops until operator click on “initialize”. Once re initialized (click on Initialize), the robot can continue the run. If the robot doesn’t succeed in initialization procedure, switch off the robot, check if needle is not clogged, move diluter (the screw under syringe) up and down and retest.

Cause : 

This error can mean that needle is blocked or diluter is damaged.

 

Corrective Task :

If the problem occurs more than 1 time each 5 runs on the same channel (which would be not plugged), diluter has to be changed. Procedure is as follows :

0- Switch off the robot, set the robotics arms in the middle in order to be able to lift the hood.

1- Unscrew bottom syringe screw

2- Put down the arm

3- Unscrew the syringe, set it on the other new diluter

4- Unscrew the 2 top tubes – BE CAREFULL OF WATER WHICH COULD DROP DOWN FROM THE 2 TUBES TO ELECTRONICS COMPONENTS OF THE ROBOT/DILUTER (you can put tubes on a wipe to absorb water.)

5- Check below the diluter if any screw retains diluter, and if there is one, unscrew it too.

6- Remove the old diluter

7- Check the address behind it, and report the same on the new one (1st diluter is “0”, 2nd is “1”, etc.)

8- Set the new one, – BE CAREFULL OF WATER WHICH COULD DROP DOWN FROM THE 2 TUBES TO ELECTRONICS COMPONENTS OF THE ROBOT/DILUTER

9- Screw firmly tubes, then syringe, then arm syringe.

10- Start robot, start Gemini and initialize. If error occurs, recheck address increment is correctly set.

 


Detection error “no liquid”

 

Cause :  

can be caused by :

–  Not enough liquid in the tube/troughs

–  Deionized liquid in the tube/trough so the robot cannot detect it.

–  ADN_SOFT underestimated the needed volume (in this case, please contact support@adnucleis.com ),

–  Evaporation of the liquid because too much time occurred between filling and program run.

 

Corrective Task

Please refill the liquid, and click on “Retry Detection”.

NB : If the volume inside looks “OK” for the estimated needed aspiration but the volume is so low that the robot can’t detect it, you can click on “Move tips to Zmax”, the robot will aspirate to the bottom of the tube / trough.

Reagents and plate setting

 

 

Slot :

 

 

Phasis :

ORIGIN ELU D1 E D2 SP W1 W2 W3 µMB DeepWell including samples on Thermoshake  

 

 

Empty 96-wells Deepwell

(And binding columns plate depending on installed technology)

Mix Tubes on Inheco with tubes adapter

 

qPCR plate on Inheco with pcr adapter

 

Extraction

ADNucleis_EXTRACT.gem or ADNucleis_EXTRACT_DIL*

Clean Waters

 

90 mL* 30 mL

 

15 mL  

X

Extraction Purification

ADNucleis_EXTRACT_PURIF.gem

All other matrices 15 mL 30 mL 15 mL Cf ADN_SOFT Cf ADN_SOFT Cf ADN_SOFT Cf ADN_SOFT Cf ADN_SOFT  

X

(The plate contains samples inside 100µL F and 100µL PVG buffers)

X
Extraction Purification COLUMNS

ADNucleis_COL_EXTRACT_PURIF.gem

All other matrices 15 mL 30 mL Cf ADN_SOFT Cf ADN_SOFT Cf ADN_SOFT Cf ADN_SOFT  

X

(The plate contains samples inside 100µL F and 100µL PVG buffers)

Seal partially the silica columns plate where unused wells :

 

 

 

X

PCR plate preparation

ADNucleis_qPCR_plate_prep.gem

Extract w/wo Purif 15 mL 30 mL  

X

(The plate contains eluted DNA Deepwell)

 

X

(cf program for ref and positions)

 

X

Automate metrology

Every Day

 

Wash the 100 mL trays :

  • For the µMB (micro Magnetic Beads) tray, if microbeads remain in the bottom, rince them with isopropanol 70% first.

  • For all tray, wash at 55°C max

 

Autoclave all the following components:

I. The washing station and pipes

II. The bottle « liquid system » and the bottle « waste ».

  

Liquide system                                                     waste

 


Every month

 

The thermoshaker has a cooling liquid inside that have to be filled every month :

  • unscrew the 2 screws

  • Mont the needle with intermediate holder :

 

  • Aspirate Inheco liquid from THERMOSHAKE COOLING LIQUID and fill it inside the Thermoshake until you can see it in the filling nozzle.